BrdU (IIB5) Antibody sc-32323 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemBrdU (IIB5) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-32323
Antibody TypePrimary Antibodies
CloneIIB5
Quantity200 µg/ml
ApplicationsIF, IHC(P), FCM
Hostmouse
Conjugate/TagUnconjugated
ClonalityMonoclonal
FormatPurified
TargetBromodeoxyuridine (BrdU)
ReactivityNSR
IsotypeIgG1

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcriptionNature CommunicationsNovember 9, 2023Diana Rubio-Contreras, Fernando Gómez-Herrerosovernight. DNA was denatured with 2 N HCl/Triton X-100. Cells were incubated with anti-BrdU (Santa Cruz, sc-32323) at 1:1000 overnight at 4 °C. After that, AlexaFluor-conjugated secondary antibody (Invitrogen) was

(2) Long non-coding RNA H19 promotes proliferation in hepatocellular carcinoma cells via H19/miR-107/CDK6 axisOncology ResearchSeptember 15, 2023ARCHITTAPON NOKKEAW, PANNATHON THAMJAMRASSRI, NAPHAT CHANTARAVISOOT, PISIT TANGKIJVANICH, CHAIYABOOT ARIYACHEThydrochloric acid prior to detection by immunofluorescence with an anti-BrdU antibody (1:200 dilution; Cat #sc-32323; Santa Cruz, Dallas, TX, United States), followed by incubation with Alexa Fluor 488-conjugated secondary

(3) The ndrg2 Gene Regulates Hair Cell Morphogenesis and Auditory Function during Zebrafish DevelopmentInternational Journal of Molecular SciencesJune 11, 2023Cheng Wang, Xin Wang, Hao Zheng, Jia Yao, Yuqing Xiang, Dong Liudilution, Abcam, #ab97959) and a mouse monoclonal anti-BrdU (1:500 dilution, Santa Cruz Biotechnology, #sc-32323, Dallas, TX, USA) were used to label the HCs, supporting cells and proliferative cells, respectively.

(4) Infection-induced extracellular vesicles evoke neuronal transcriptional and epigenetic changesScientific ReportsApril 27, 2023Ellen Tedford, Norhidayah Binti Badya, Conor Laing, Nozomi Asaoka, Shuji Kaneko, Beatrice Maria Filippi, Glenn Alan McConkeyimmunoprecipitation was performed with protein G Dynabeads and IgG anti-BrdU antibody IIB5 (Santa Cruz Biotechnology, SC-32323). RNA was extracted and RT-qPCR performed as previously described. Methylation sensitive restriction

(5) FAM193A is a positive regulator of p53 activityCell ReportsMarch 28, 2023Maria M. Szwarc, Anna L. Guarnieri, Molishree Joshi, Huy N. Duc, Madison C. Laird, Ahwan Pandey, Santosh Khanal, Emily Dohm, Aimee K. Bui, Kelly D. Sullivan, et al.RRID:AB_10695870 BID,Cell Signaling Technology,Cat#2002; RRID:AB_10692485 BrdU,Santa Cruz Biotechnology,Cat#sc-32323; RRID:AB_626766 Cleaved Caspase-3,Cell Signaling Technology,Cat#9661; RRID:AB_2341188 Caspase-8,Cell Signaling Technology

(6) Hindering NAT8L expression in hepatocellular carcinoma increases cytosolic aspartate delivery that fosters pentose phosphate pathway and purine biosynthesis promoting cell proliferationRedox BiologyFebruary 1, 2023Pamela De Falco, Giacomo Lazzarino, Federica Felice, Enrico Desideri, Serena Castelli, Illari Salvatori, Fabio Ciccarone, Maria Rosa Ciriolocell counting. For BrdU incorporation assay, cells were incubated with 10 μM BrdU (Santa Cruz Biotechnology, sc-32323) for 4 h and fixed for 30 min with 90% ethanol, 5% acetic acid, 5% water fix solution. DNA denaturation

(7) Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELFNature CommunicationsDecember 1, 2022Vladyslava Gorbovytska et al.previously described70. Nascent RNA was immunoprecipitated with anti-BrdU antibody-conjugated beads (sc-32323 AC, Santa Cruz Biotech, Santa Cruz, CA). Purified run-on RNA was subjected to polyA tailing by Poly(A)-polymerase

(8) Trp53 controls chondrogenesis and endochondral ossification by negative regulation of TAZ activity and stability via β-TrCP-mediated ubiquitinationCell Death DiscoveryDecember 1, 2022Yang Li, Shu-ting Yang, Shuying Yanginsulin-transferrin-sodium selenite media supplement (ITS Supplement) were purchased from Cell Signaling Technology. BrdU (#sc-32323; dilution 1:1000), β-TrCP (#sc-390629; dilution 1:1000), GFP (#sc-9996; dilution 1:1000) and flag (#sc-7945;

(9) Targeting the Erk1/2 and autophagy signaling easily improved the neurobalst differentiation and cognitive function after young transient forebrain ischemia compared to old gerbilsCell Death DiscoveryDecember 1, 2022Fuxing Wang, Zihao Xia, Peng Sheng, Yu Ren, Jiajia Liu, Lidong Ding, Bing Chun YanSanta Cruz Biotechnology, cat. sc-8066), and mouse anti-BrdU (1:100, Santa Cruz Biotechnology, cat. sc-32323) at room temperature for 12 h. Subsequently, they were treated with the corresponding secondary antibodies,

(10) Keratin-mediated hair growth and its underlying biological mechanismCommun BiolNovember 19, 2022Seong Yeong An, Hyo-Sung Kim, So Yeon Kim, Se Young Van, Han Jun Kim, Jae-Hyung Lee, Song Wook Han, Il Keun Kwon, Chul-Kyu Lee, Sun Hee Do, et al.staining was done by immunocytochemical staining with mouse anti-BrdU antibody (Santa Cruz Biotechnology, sc-32323, diluted 1:100). In addition, BrdU incorporation assay was done by BrdU Cell Proliferation ELISA Kit

(11) The androgen receptor couples promoter recruitment of RNA processing factors to regulation of alternative polyadenylation at the 3' end of transcriptsNucleic Acids ResearchSeptember 23, 2022Cinzia Caggiano, Marco Pieraccioli, Consuelo Pitolli, Gabriele Babini, Dinghai Zheng, Bin Tian, Pamela Bielli, Claudio SetteRNasin ribonuclease inhibitors (N2518, Promega), and incubated with 1.25 μg of anti-BrdU antibody (sc-32323, Santa Cruz Biotechnology) in the presence of Dynabeads protein G (Invitrogen) at 4°C for 2 h. The bead–antibody–RNA

(12) Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seqSTAR ProtocolsSeptember 16, 2022Yan Chen, Jiafu Zhu, Yilin Xie, Zijuan Li, Yijing Zhang, Min Liu, Zhicheng DongRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, BrdU (IIB5): mouse monoclonal IgG (3:100),Santa Cruz Biotechnology,sc-32323 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(13) Circular EZH2-encoded EZH2-92aa mediates immune evasion in glioblastoma via inhibition of surface NKG2D ligandsNature communicationsAugust 15, 2022Zhong J, Yang X, Chen J, He K, Gao X, Wu X, Zhang M, Zhou H, Xiao F, An L, et al.kit (AM1907, Thermo Fisher Scientific). The NRO-RNA was incubated with anti-BrdU antibody (2 μg/tube, SC-32323, clone IIB5, Santa Cruz)-coated Protein G beads for 30 min at room temperature. The beads were captured

(14) GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosisCell & BioscienceAugust 6, 2022Xiaoni Shi, Jing Wang, Xinxin Zhang, Shaoqi Yang, Wei Luo, Sha Wang, Jie Huang, Mengling Chen, Yusi Cheng, Jie Chaowith goat serum at room temperature for 2 h. The cells were incubated with a BrdU antibody (1:100; SC-32323, Santa Cruz) at 4 °C overnight. After washing with PBS, the cells were incubated with appropriate fluorescent

(15) IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolismRedox BiologyAugust 1, 2022Yang Li, Shuting Yang, Yang Liu, Ling Qin, Shuying Yangkits and 2-Deoxy-d-glucose (2-DG) were ordered from Sigma. Antibodies against MMP13 (sc-515284), BrdU (sc-32323), ColX (sc-59954), actin (sc-47778), Lamin (sc-377000) and Smad2/3 (sc-133098) were obtained from Santa Cruz Biotechnology

(16) Endothelial cell-derived extracellular vesicles impair the angiogenic response of coronary artery endothelial cellsFrontiers in Cardiovascular MedicineJuly 19, 2022Nigeste Carter, Allison H. Mathiesen, Noel Miller, Michael Brown, Ruben M. L. Colunga Biancatelli, John D. Catravas, Anca D. Dobriancells were incubated with anti-BrdU Alexa Fluor 488 antibody (Santa Cruz Biotechnology, catalog no. SC-32323 AF488) and 5 μg/mL DAPI prepared in 10% NGS. Wells were washed 3× with PBS, and coverslipped with Fluoromount-G.

(17) WDR62 variants contribute to congenital heart disease by inhibiting cardiomyocyte proliferationClinical and Translational MedicineJuly 8, 2022Lili Hao et al.embryos were incubated with the rabbit anti‐Histone H3 (phospho S10) (ab5176, Abcam) and mouse anti‐BrdU (sc‐32323, Santa Cruz Biotechnology). For BrdU assay, embryos were exposed to BrdU for 6 h before collection. For

(18) Paternal Radiofrequency Electromagnetic Radiation Exposure Causes Sex-Specific Differences in Body Weight Trajectory and Glucose Metabolism in Offspring MiceFrontiers in Public HealthMay 6, 2022Song Yan, Ying Ju, Jie Dong, Hui Lei, Jun Wang, Qian Xu, Yefei Ma, Jingjing Wang, Xiaohong Wangthrice with 1.5 M Tris-HCl (pH = 8.8). Slides were incubated with primary antibodies (BrdU, Santa Cruz, sc-32323; 1:100) overnight at 4°C, and subsequently incubated with Alexa Fluor 488 anti-mouse secondary antibody

(19) FAK PROTAC Inhibits Ovarian Tumor Growth and Metastasis by Disrupting Kinase Dependent and Independent PathwaysFrontiers in OncologyApril 28, 2022Xueyun Huo, Wenjing Zhang, Guannan Zhao, Zhenwen Chen, Peixin Dong, Hidemichi Watari, Ramesh Narayanan, Todd D. Tillmanns, Lawrence M. Pfeffer, Junming Yuefixed with 3.7% formalin and cells were washed and incubated with BrdU antibody (Santa Cruz, Cat.No. SC-32323) for 24h at 4°C and then fluorescent labelled secondary antibody Alexa 488 (Thermo Fisher Scientific.

(20) Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency☆HeliyonMarch 15, 2022Guanxu Ji, Xiaoxiao Xiao, Min Huang, Qiang Wuparaformaldehyde (PFA), washed with PBS. The primary antibody used to probe the cells was anti-BrdU (sc-32323, Santa Cruz) while the secondary antibody used was Alexa Fluor 594 lgG antibody (Invitrogen). 2.7 Gene expression microarray analysis

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BrdU (IIB5) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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BrdU (IIB5) Antibody from Santa Cruz Biotechnology, Inc.

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